解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We describe a new approach for the characterization of a digested protein complex with quantitative aspects. Accurate masses of tryptic peptides in the digested complex were acquired by nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (MS). The conditions of the electrospray ion s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300812
更新日期:2004-09-01 00:00:00
abstract::The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300754
更新日期:2004-08-01 00:00:00
abstract::The pilot phase of the Human Brain Proteome Project as a part of the Human Proteome Organisation has just been started. In two pilot studies, 18 different laboratories are analyzing mouse brains of three age stages and human brain autopsy versus biopsy material, respectively. The overall aim is to elucidate the portfo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400867
更新日期:2004-08-01 00:00:00
abstract::Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300854
更新日期:2004-08-01 00:00:00
abstract::During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300723
更新日期:2004-07-01 00:00:00
abstract::Phage display techniques using random peptide interactions have supported the role of mammalian glutathione transferase (GST) as part of a signalling pathway for both oxidative stress and an apoptosis pathway. Little is known about the interaction of nonmammalian GST with other proteins. GSTs have been implicated in t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300719
更新日期:2004-07-01 00:00:00
abstract::Adipogenesis is an important aspect of energy homeostasis. Here we have used a differential proteome mapping strategy to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3 L1 preadipocytes. Two-dimensional gel electrophoresis analysis identified 8 proteins that are induc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300697
更新日期:2004-06-01 00:00:00
abstract::Since the identification of approximately 25,000 proteins from the draft human genome assembly in 2001, estimates of the total have oscillated between 30,000 and 70,000. The recently announced genome closure has not generated a consensus gene count despite this being a key parameter for many areas of biology including...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300700
更新日期:2004-06-01 00:00:00
abstract::A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K-12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well-documented Gram-negative proteome of E. coli K-12 was chosen to assess how the different approaches complement each oth...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300749
更新日期:2004-06-01 00:00:00
abstract::Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300736
更新日期:2004-05-01 00:00:00
abstract::Voltage-dependent anion channel (VDAC) proteins are small, abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apopt...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300734
更新日期:2004-05-01 00:00:00
abstract::A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300678
更新日期:2004-04-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300672
更新日期:2004-04-01 00:00:00
abstract::To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300653
更新日期:2004-04-01 00:00:00
abstract::Two-dimensional difference gel electrophoresis (2-D DIGE) coupled with mass spectrometry (MS) was used to investigate tumor-specific changes in the proteome of human colorectal cancers and adjacent normal mucosa. For each of six patients with different stages of colon cancer, Cy5-labeled proteins isolated from tumor t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300635
更新日期:2004-03-01 00:00:00
abstract::A method for the determination of the stoichiometry of protein complexes has been developed, which is based on proteolytic digestion of the complex, labeling with a fluorescent reagent, specific for amino or sulfhydryl groups, and separation by liquid chromatography with fluorescence and mass spectrometric detection. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300668
更新日期:2004-03-01 00:00:00
abstract::A two-dimensional liquid-phase separation scheme coupled with mass spectrometry (MS) is presented for proteomic analysis of cell lysates from normal and malignant breast epithelial cell lines. Liquid-phase separations consist of isoelectric focusing as the first dimension and nonporous silica reverse-phase high-perfor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300606
更新日期:2004-03-01 00:00:00
abstract::Uni- or multidimensional microcapillary liquid chromatography (microLC) matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry (MS/MS) approaches have gained significant attention for quantifying and identifying proteins in complex biological samples. The off-line coupling of microLC with MS quan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300566
更新日期:2004-02-01 00:00:00
abstract::The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300645
更新日期:2004-02-01 00:00:00
abstract::Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300630
更新日期:2004-02-01 00:00:00
abstract::The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300548
更新日期:2004-01-01 00:00:00
abstract::A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300543
更新日期:2004-01-01 00:00:00
abstract::Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300616
更新日期:2003-12-01 00:00:00
abstract::We compared peptide identification by database (DB) search methods with de novo sequencing results for proteomics study in an organism without genome sequence information. When the former was done by searching the Expressed Sequence Tag (EST) DB of the sample organism or the NCBI nonredundant (nr) protein DB of green ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300620
更新日期:2003-12-01 00:00:00
abstract::The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300581
更新日期:2003-11-01 00:00:00
abstract::Antibody microarrays have great potential for significant value in biological research. Cancer research in particular could benefit from the unique experimental capabilities of this technology. This article examines the current state of antibody microarray technological developments and assay formats, along with a rev...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300595
更新日期:2003-11-01 00:00:00
abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300465
更新日期:2003-10-01 00:00:00
abstract::The senescence-accelerated mouse (SAM) is a useful animal model to study aging or age-associated disorders due to its inherited aging phenotype. To investigate proteins involved in the aging process in liver, we compared the young (4- or 20-week old) and the aged group (50-week-old) of SAMP8 (short life span) and SAMR...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300562
更新日期:2003-10-01 00:00:00
abstract::We bring a "spectrum" of classical data mining and statistical analysis methods to bear on discrimination of two groups of spectra from 24 diseased and 17 normal patients. Our primary goal is to accurately estimate the generalizability of this small dataset. After an aggressive preprocessing step that reduces consider...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300516
更新日期:2003-09-01 00:00:00
abstract::In order to proceed through their life cycle, protozoan parasites of the genus Leishmania cycle between sandflies and mammals. This change of environment correlates with the differentiation from the promastigote stage (insect form) to the amastigote stage (intracellular mammalian form). The molecular basis underlying ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300462
更新日期:2003-09-01 00:00:00
abstract::We monitored rates of growth, ATP-synthesis, respiration, and death to assess the sensitivity of the model organism Pseudomonas putida KT2440 to methyl tert-butyl ether (MTBE), and its degree of toxicity. The physiological data obtained suggested that the energy conservation system was the most sensitive site. However...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300477
更新日期:2003-08-01 00:00:00
abstract::By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300488
更新日期:2003-08-01 00:00:00
abstract::The solubilization of a large array of proteins before electrophoresis itself is a very critical point for proteomic analyses. We compared the efficiency of several different solubilization buffers. From this work, we defined a very efficient solubilization buffer, including two chaotropes, two reducing agents (R2), t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300450
更新日期:2003-07-01 00:00:00
abstract::Unfortunately, conventional large-format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for visualization of the proteins. This problem is compounded when large-format two-dimensional gels are subjected to multiple staining procedures in order to detect differe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300440
更新日期:2003-07-01 00:00:00
abstract::The recombinant plasmid pASK18 carries a Streptomyces DNA fragment which includes an open reading frame, designated psfS (putative sigma factor, Streptomyces), as its putative product showed a high degree of similarity with RNA polymerase sigma factors. Previous results showed that PsfS causes transcription initiation...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300403
更新日期:2003-06-01 00:00:00
abstract::Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300375
更新日期:2003-06-01 00:00:00
abstract::In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300402
更新日期:2003-06-01 00:00:00
abstract::The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300409
更新日期:2003-05-01 00:00:00
abstract::Many of the emerging technologies for the global evaluation of gene expression, at both the RNA and protein level, are being applied to the problem of finding biomarkers for human disease progression. These analyses can be made difficult, however, by variation between samples that arises from both technical and nondis...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300399
更新日期:2003-05-01 00:00:00
abstract::Lipid rafts are membrane microdomains of unique lipid composition that segregate proteins with poorly understood consequences for membrane organization. Identification of raft associated proteins could therefore provide novel insight into raft-dependent functions. Monocytes process antigens for presentation to T cells...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200390067
更新日期:2003-04-01 00:00:00